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plasmid 1154b  (Addgene inc)


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    Structured Review

    Addgene inc plasmid 1154b
    Plasmid 1154b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+1154b/plasmid+gfle+1154b/pm37406109-169-2-17
    Average 90 stars, based on 1 article reviews
    plasmid 1154b - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: A confinable female-lethal population suppression system in the malaria vector, Anopheles gambiae
    Article Snippet: We modified a piggyBac transgenesis backbone pbVTKactR ( ) to contain each gRNA under expression of the A. gambiae U6 promoter ( ) synthesized as gBlocks®, and replaced endogenous Red for m2Turquoise (ECFP) The final plasmid sequence (plasmid 1154B, transgene gFLE) was confirmed by Sanger sequencing and is available on Addgene (#187238).

    Article Title: A confinable female-lethal population suppression system in the malaria vector, Anopheles gambiae .
    Article Snippet: The final plasmid sequence (plasmid 1154B, transgene gFLE) was confirmed by Sanger sequencing and is available on Addgene (no. 187238).

    Sequencing:

    Article Title: A confinable female-lethal population suppression system in the malaria vector, Anopheles gambiae
    Article Snippet: We modified a piggyBac transgenesis backbone pbVTKactR ( ) to contain each gRNA under expression of the A. gambiae U6 promoter ( ) synthesized as gBlocks®, and replaced endogenous Red for m2Turquoise (ECFP) The final plasmid sequence (plasmid 1154B, transgene gFLE) was confirmed by Sanger sequencing and is available on Addgene (#187238).

    Article Title: A confinable female-lethal population suppression system in the malaria vector, Anopheles gambiae .
    Article Snippet: The final plasmid sequence (plasmid 1154B, transgene gFLE) was confirmed by Sanger sequencing and is available on Addgene (no. 187238).

    Modification:

    Article Title: A confinable female-lethal population suppression system in the malaria vector, Anopheles gambiae
    Article Snippet: We modified a piggyBac transgenesis backbone pbVTKactR ( ) to contain each gRNA under expression of the A. gambiae U6 promoter ( ) synthesized as gBlocks®, and replaced endogenous Red for m2Turquoise (ECFP) The final plasmid sequence (plasmid 1154B, transgene gFLE) was confirmed by Sanger sequencing and is available on Addgene (#187238).

    Article Title: A confinable female-lethal population suppression system in the malaria vector, Anopheles gambiae .
    Article Snippet: The final plasmid sequence (plasmid 1154B, transgene gFLE) was confirmed by Sanger sequencing and is available on Addgene (no. 187238).

    Expressing:

    Article Title: A confinable female-lethal population suppression system in the malaria vector, Anopheles gambiae
    Article Snippet: We modified a piggyBac transgenesis backbone pbVTKactR ( ) to contain each gRNA under expression of the A. gambiae U6 promoter ( ) synthesized as gBlocks®, and replaced endogenous Red for m2Turquoise (ECFP) The final plasmid sequence (plasmid 1154B, transgene gFLE) was confirmed by Sanger sequencing and is available on Addgene (#187238).

    Article Title: A confinable female-lethal population suppression system in the malaria vector, Anopheles gambiae .
    Article Snippet: The final plasmid sequence (plasmid 1154B, transgene gFLE) was confirmed by Sanger sequencing and is available on Addgene (no. 187238).

    Synthesized:

    Article Title: A confinable female-lethal population suppression system in the malaria vector, Anopheles gambiae
    Article Snippet: We modified a piggyBac transgenesis backbone pbVTKactR ( ) to contain each gRNA under expression of the A. gambiae U6 promoter ( ) synthesized as gBlocks®, and replaced endogenous Red for m2Turquoise (ECFP) The final plasmid sequence (plasmid 1154B, transgene gFLE) was confirmed by Sanger sequencing and is available on Addgene (#187238).

    Article Title: A confinable female-lethal population suppression system in the malaria vector, Anopheles gambiae .
    Article Snippet: The final plasmid sequence (plasmid 1154B, transgene gFLE) was confirmed by Sanger sequencing and is available on Addgene (no. 187238).



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    plasmid 1154b  (Addgene inc)
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    Addgene inc plasmid 1154b
    Plasmid 1154b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+1154b/plasmid+gfle+1154b/pm37406109-169-2-17
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    plasmid gfle 1154b  (Addgene inc)
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    Addgene inc plasmid gfle 1154b
    ( A ) The <t>gFLE</t> <t>transgene</t> expresses two gRNAs (gRNA7 and gRNA10, teal and emerald, respectively) targeting fle near the start codon and within the first RNA recognition motif (RRM), respectively (gene map, indigo). The PolIII U6 promoter facilitates gRNA expression (navy), selection is enabled by Act-EGFP-Sv40 (purple, green, and red), and the transgene was inserted by piggyBac transgenesis [terminal repeats (TR), gray arrows]. The Cas9 transgene expresses Cas9 in the adult germline and is maternally deposited into the embryo and was previously described by Werling et al. . ( B ) Individual genotypes are identified by fluorescence with Cas9 marked by 3xP3-DsRed and gFLE marked by Act5c-GFP. ( C ) Crossing F0 gFLE/+ males to Cas9/+ females yields F1 offspring in Mendelian 1:1:1:1 ratios, with the gFLE/Cas9 female cohort absent. ( D ) The absence of all F1 gFLE/Cas9 and some gFLE/+ female offspring suggests that fle mutagenesis results in female death. This effect is heritable into the F2 and F3 generations with gFLE G /Cas9 males mated to +/+ females able to kill daughters with the appropriate genotypes (F2 and F3 data from gFLE I /Cas9 and gFLE J /Cas9 males reported in fig. S10). ( E ) Crossing homozygous gFLE G /gFLE G to Cas9/Cas9 results in complete elimination of adult genetic daughters from the progeny regardless of parental sex.
    Plasmid Gfle 1154b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+1154b/plasmid+gfle+1154b/pmc10321730-197-13-15
    Average 90 stars, based on 1 article reviews
    plasmid gfle 1154b - by Bioz Stars, 2026-09
    90/100 stars
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    Image Search Results


    ( A ) The gFLE transgene expresses two gRNAs (gRNA7 and gRNA10, teal and emerald, respectively) targeting fle near the start codon and within the first RNA recognition motif (RRM), respectively (gene map, indigo). The PolIII U6 promoter facilitates gRNA expression (navy), selection is enabled by Act-EGFP-Sv40 (purple, green, and red), and the transgene was inserted by piggyBac transgenesis [terminal repeats (TR), gray arrows]. The Cas9 transgene expresses Cas9 in the adult germline and is maternally deposited into the embryo and was previously described by Werling et al. . ( B ) Individual genotypes are identified by fluorescence with Cas9 marked by 3xP3-DsRed and gFLE marked by Act5c-GFP. ( C ) Crossing F0 gFLE/+ males to Cas9/+ females yields F1 offspring in Mendelian 1:1:1:1 ratios, with the gFLE/Cas9 female cohort absent. ( D ) The absence of all F1 gFLE/Cas9 and some gFLE/+ female offspring suggests that fle mutagenesis results in female death. This effect is heritable into the F2 and F3 generations with gFLE G /Cas9 males mated to +/+ females able to kill daughters with the appropriate genotypes (F2 and F3 data from gFLE I /Cas9 and gFLE J /Cas9 males reported in fig. S10). ( E ) Crossing homozygous gFLE G /gFLE G to Cas9/Cas9 results in complete elimination of adult genetic daughters from the progeny regardless of parental sex.

    Journal: Science Advances

    Article Title: A confinable female-lethal population suppression system in the malaria vector, Anopheles gambiae

    doi: 10.1126/sciadv.ade8903

    Figure Lengend Snippet: ( A ) The gFLE transgene expresses two gRNAs (gRNA7 and gRNA10, teal and emerald, respectively) targeting fle near the start codon and within the first RNA recognition motif (RRM), respectively (gene map, indigo). The PolIII U6 promoter facilitates gRNA expression (navy), selection is enabled by Act-EGFP-Sv40 (purple, green, and red), and the transgene was inserted by piggyBac transgenesis [terminal repeats (TR), gray arrows]. The Cas9 transgene expresses Cas9 in the adult germline and is maternally deposited into the embryo and was previously described by Werling et al. . ( B ) Individual genotypes are identified by fluorescence with Cas9 marked by 3xP3-DsRed and gFLE marked by Act5c-GFP. ( C ) Crossing F0 gFLE/+ males to Cas9/+ females yields F1 offspring in Mendelian 1:1:1:1 ratios, with the gFLE/Cas9 female cohort absent. ( D ) The absence of all F1 gFLE/Cas9 and some gFLE/+ female offspring suggests that fle mutagenesis results in female death. This effect is heritable into the F2 and F3 generations with gFLE G /Cas9 males mated to +/+ females able to kill daughters with the appropriate genotypes (F2 and F3 data from gFLE I /Cas9 and gFLE J /Cas9 males reported in fig. S10). ( E ) Crossing homozygous gFLE G /gFLE G to Cas9/Cas9 results in complete elimination of adult genetic daughters from the progeny regardless of parental sex.

    Article Snippet: To identify transgene insertion sites, nanopore reads were aligned to plasmids carrying either gFLE (1154B, Addgene, as plasmid no. 187238) or Cas9 ( ) constructs using minimap2 ( ).

    Techniques: Expressing, Selection, Fluorescence, Mutagenesis